Abstract
Serotonin transporter (5-HTT) mRNA and 5-HTT sites were measured 3 days after treatment with p-chlorophenylalanine, a tryptophan hydroxylase inhibitor. While 5-HTT mRNA levels decreased (P < 0.001) in the dorsal raphe nucleus, 5-HTT binding sites remained unchanged, suggesting that an acute depletion of 5-HT may induce an increase in the turnover of 5-HTT mRNA without affecting protein levels at 3 days.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Carrier Proteins / genetics*
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Down-Regulation
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Enzyme Inhibitors / pharmacology
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Fenclonine / pharmacology
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Histocytochemistry
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In Situ Hybridization
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Male
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Membrane Glycoproteins / genetics*
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Membrane Transport Proteins*
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Nerve Tissue Proteins / genetics*
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Neurons / drug effects*
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Neurons / metabolism
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RNA, Messenger / metabolism*
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Raphe Nuclei / cytology
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Raphe Nuclei / drug effects*
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Raphe Nuclei / metabolism
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Rats
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Rats, Sprague-Dawley
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Serotonin / metabolism*
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Serotonin Agents / pharmacology
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Serotonin Plasma Membrane Transport Proteins
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Tryptophan Hydroxylase / antagonists & inhibitors
Substances
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Carrier Proteins
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Enzyme Inhibitors
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Membrane Glycoproteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Serotonin Agents
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Serotonin Plasma Membrane Transport Proteins
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Slc6a4 protein, rat
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Serotonin
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Tryptophan Hydroxylase
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Fenclonine